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HPLC helps determine the percentage of the desired peptide present in the final product while identifying potential impurities that could affect experimental outcomes

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Don't microwave, don't use warm water, and never shake the peptide vial after adding the water

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Despite these scalability trade-offs, Sortase A has successfully enabled the dense display of SARS-CoV-2 RBD on PapMV VLPs (Olivera-Ugarte et al., 2022) and EV71 epitopes on HBc particles (Tang et al., 2016), eliciting robust humoral responses