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Using averaged metrics from our laboratory records where adult, male rats weighed 300 grams and consumed 22 grams of chow per day, we modified AIN-76A Purified Rodent Diet (Dyets, Inc., Bethelehem, PA) with 4.0% NaCl and 0.82 grams of Protandim per kilogram of chow, for a final dose of approximately 60.5 mg/kg/day or 450 mg/m 2

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doi: 10.1097/01.TP.0000129260.86766.67 12 BrayRAGebelHM

The microbiome-gut-brain content axis consists of three bidirectional, biochemical, signaling pathways between the gut and the brain, and these three pathways include the nervous, endocrine, and immune pathways (198)