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The treated cells were lysed with radioimmunoprecipitation assay (RIPA) buffer [50 mM TrisHCl (pH 8.0), 0.05 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) SDS, and 150 mM NaCl] containing protease inhibitor cocktails (Merck, USA) with a ratio of 1:1000

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89 In conclusion, BPC-157 offers a compelling case study in modern biomedical science: a promising therapy with loads of preclinical support and an eager fan base, standing on the threshold of mainstream acceptance but held back by unanswered questions

Chen, B., Wang, J