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The traction tests that followed were performed in the following conditions: rectangular geometry, isothermal for 5 min, 30C, and ramp force 0.2 N/min from 0.001 N to 18 N ( 2.4.2 Dispersion of biogenic SiNPs For the dispersion of SiNPs in alginate, we used probe sonication (Sonics VCX 750, model CV334, Sonics & Materials, Newtown, CT, USA) with the following parameters: 20 kHz, 5 min, 80% amplitude, pulse 15 sec, and pause 30 sec (Retamal Marn et al., 2017

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Research has demonstrated that GA modulates the AMPK/SREBP/ACC and IRs-1/Akt signaling pathways, which are pivotal in cellular metabolism, and it also suppresses liver inflammation by targeting the IKK/i-B/NF-B signaling pathway, a key mediator of inflammatory responses (L et al., 2023)

By reducing SAM consumption through NNMT inhibition, 5-Amino-1MQ helps preserve methylation capacity for other critical cellular processes, including DNA and histone methylation (epigenetic regulation), neurotransmitter synthesis, and phospholipid metabolism

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