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THP-1 cells, a human acute monocytic leukemic cell line, were obtained from ATCC (TIB-202) and cultured in an incubator at 37 C, 5% CO 2 , and 95% humidity in RPMI-1640 medium containing 2 mM L-glutamine, 25 mM HEPES, 1% sodium pyruvate, 0.01% of 2-mercaptoethanol (2-ME), 10% fetal bovine serum (FBS), and 1% penicillin/streptomycin