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Therapeutic targeting of the mevalonate-geranylgeranyl diphosphate pathway with statins overcomes chemotherapy resistance in small cell lung cancer

Koppers et al

doi: 10.3390/ijms20040974 14 WuerchEUrgoitiGRYongVW

The beads were then washed twice with binding/wash solution, bound to the target antibody, reacted on ice for 2 h, added protein samples, and kept on ice overnight on a shaker

The concentration was determined according to a standard curve as follows: ATP (M) =( R sample R blank/ Slope ( M -1 )) x DF where RSample = Luminescence (RLU) value of the sample, RBlank = Luminescence (RLU) value of the blank, DF = Sample dilution factor (DF = 1 for undiluted samples) obtained from ATP standards according to the manufacturers recommendation

25 Methylfolate and methylcobalamin are critical for the metabolism of homocysteine via the remethylation pathway, and NAC works alongside these nutrients in countering oxidative stress