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Standalone 5-Amino-1MQ without dietary or pharmacological caloric reduction will have more modest effects

A: L-Glutathione reduced powder can be synthesized through fermentation-based biosynthesis using yeast or engineered microbes, chemical reduction of oxidized glutathione, or electrochemical reduction techniques

Acknowledgments This work was supported by the Natural Science Foundation of Jiangsu Province

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Result: In the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM -ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents