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reported that LKB1 mutation in KRAS mutant lung adenocarcinoma confers a glutamate enriched phenotype in TME, and this feature was associated with CD8 + T cell activation against PD-1, whereas treatment with the glutaminase inhibitor CB-839 inhibited CD8 + T cell expansion and activation

10.1155/2020/5849794 104 WangY

Proteins (30 g) underwent electrophoresis on 12% SDS-PAGE gels, transferred onto PVDF membranes (Millipore, United States), and blocked with QuickBlock Blocking Buffer (Beyotime, P0252) for 1 h

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After incubating for 24 h at 37 C, the medium was collected, and formaldehyde was quantified

Clinical trials guiding evidence for treatment are lacking due to the rarity of disease, heterogeneous clinical course, and lack of prognostic biomarkers, all of which contribute to difficulty in clinical trial design and implementation