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HC neutrophils were cultured in the presence of HC or SLE serum with or without the addition of Cl-amidine (Cl) (peptidyl arginine deiminase 4 (PAD4) inhibitor, 100 M), or LPX-1 (1 M) for 4 or 16 hours and NETs were assessed in SYTOX Green+ cells based on morphology (n = 6)

In contrast, tissue infected with pyocyanin-deficient strains does not exhibit this accumulation (108)

The hotplate latencies were normalized to the percentage of the MPE using the following formula: % MPE = 100 (post-injection latency pre-injection baseline latency)/(maximum latency 60 s pre-injection baseline latency)

Lau, A., Kennedy, B

Fluorescence was measured on a microplate reader at 485 nm excitation and 520 nm emission for CellROX, 485/535 nm Ex/Em for DCFDA

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