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this activation can increase the expression of proinflammatory cytokines such as IL-1, IL-6, TNF- and COX-2 to regulate the inflammatory response and unbalance the MMPs/tissue inhibitors of metalloproteinases (TIMPs) ratio by activating MMPs and reducing TIMPs expression, thereby decompressing the ECM (6,31,41)

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The samples were then blocked in protein block serum (Agilent) with 0.3% Triton X-100 (Thermo Fisher) for 1 h

Magnesium is responsible for over 600 enzymatic functions in the body and is an important co-factor you want to have onboard