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10.1016/j.watres.2013.08.024 32 LuoY.GuoW.NgoH

UPF, which consists of carbohydrates and fats, have several important functions which lead to several synergistic actions which alter the brain's reward system (121)

Salas-Salvad, J., Mrquez-Sandoval, F., & Bull, M

By stimulating cellular function and encouraging improved nutrient delivery to the follicles, peptides assist in strengthening the hair's internal structure, rendering it more resistant to outside stressors such as heat, pollution, and styling damage

In parallel, capsaicin induces the expression of antimicrobial peptides, such as regenerating islet-derived protein 3 gamma (Reg3), and modulates bile acid metabolism, both of which contribute to maintaining microbial diversity and community stability (120, 143, 145)

Following methanol-chloroform precipitation, proteins were denatured and reduced in 6 M urea, 2 M thiourea, and 10 mM tris(2-carboxyethyl) phosphine (TCEP, ThermoFisher)