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This activation triggers a cascade of downstream signaling events leading to the activation of the PI3K-AKT/PKB and Ras-MAPK pathways

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Effect on cell viability was assessed 72 h after transfection with GSTO1 siRNA or siSCRAM by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and normalized to mock-transfected control (transfection reagent)