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Once hair follicles become sensitive to DHT, the genetic tendency toward hair loss does not disappear

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ATP-Glo bioluminometric cell viability assay In total, 1,000 cells (Pfa1) or 10,000 cells (HeLa and U2OS) per well were seeded into white opaque 96-well plates (136101, Thermo Fisher Scientific)

Statistical analysis Statistical analysis was done using one tailed students t-test for pair wise comparison or ANOVA followed by post-hoc Tukey test for multiple sample comparisons

An auto-regulatory loop between stress sensors INrf2 and Nrf2 controls their cellular abundance