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10.1016/j.heliyon.2020.e03411 98 OdehD.OroliN.BerendikaM.ikiD.Domjani DrozdekS.BalbinoS.et al (2022)

10.1016/j.biopha.2021.112419 105 MalikA

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Transfected COS7 cells were washed and lysed in lysis buffer (1% Triton X-100, 20 mM Tris-HCl [Ph 8], 130 mM NaCl, 10 mM NaF, 1% aprotonin, 10 g/ml leupeptin, 1 mM dithiothreitol (43816, DTT), 0.1 mM Na 3 VO 4 and 1 mM phenylmethylsulfonyl fluoride (PMSF))

Indeed, that any deviation from the recommended eight hours sleep duration over time was associated with a biomarker of brain inflammation aligns with prior reports of U-shaped sleep duration-health relationships, including elevated risk of cardiovascular diseases, metabolic disorders, and mortality [43], as well as markers of amyloid deposition in cerebrospinal fluid (CSF) [48]