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Weaning Off the Walking Boot Before swapping the bulky boot for regular shoes, confirm all three of these boxes: Stand on the injured leg in the boot for 10 seconds without a wobble Pain 2/10 with full body weight through the boot Dorsiflexion within 10 of the opposite side when measured by your PT Typical wedge removal schedule Remove the final heel wedge at Week 6 and lock the boot in neutral Week 78: Boot on indoors, athletic shoe with a -inch heel lift outdoors for short distances Week 910: Shoe inside and out

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A later study using isobaric tag for relative and absolute quantitation (iTRAQ) and one-dimensional gel electrophoresis-Fourier transform mass spectrometry/ion trap tandem mass spectrometry (FTMS/ITMSMS) that compared normal and degenerative discs showed that in patient samples, 73 proteins in the AF and 54 proteins in the NP exhibited differential expression levels between the two groups (Sarath Babu et al., 2016)