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The Formulation Challenge Combining these two ingredients in a stable, effective serum is not straightforward

Cell suspension was plated onto poly-D,L-ornithine-coated plates with DMEM, 1ITS (Life Technologies), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.5% FBS and 0.05% gentamicin (Sigma), 10 ng/ml PDGF and 10 ng/ml FGF (Peprotech), with full medium change the next day and half medium change every other day

2 General characteristics of PDEV 2.1 Naming and classification of PDEV According to the guidelines established by the International Society for Extracellular Vesicles (ISEV), the term extracellular vesicles (EVs) is recommended as the standardized nomenclature to describe naturally secreted particles that are delimited by a lipid bilayer and lack the capacity for self-replication (Witwer and Thery, 2019)

Salim, A

Piccioni, D

Clinically, symptoms develop initially in the feet and hands, followed by proximal progression to the ankles and wrists in a stocking and glove distribution (Lomonaco et al., 1992