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Polyclonal antibodies against protein kinase B (Akt), phosphorylated protein kinase B (p-Akt), AMPK, p-AMPK, NFkB-p65, IkB, Nrf2, VDAC, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology, Inc

GSSG is then reduced back into GSH by the activity of glutathione reductase, which requires NADPH to regenerate the mitochondrial pool of GSH (reviewed in greater detail in Andreyev et al

Since tumor cells with certain oncogenic mutations are very sensitive to ferroptosis, triggering ferroptosis may also have significant therapeutic potential for ferroptosis-sensitive tumor cells ( c /GSH/GPX4 axis have been shown to be fatal in host cells

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The frozen pooled samples were homogenized in a phosphate-buffered solution (pH=6.8) at a 1:10 proportion (weight:volume) in an ice-cooled container

rather, they are a diverse group of molecules that are responsible for a wide range of biological functions [8]