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Membranes were blocked with TBS + 0.1% (v/v) Tween 20 (TBST) and 5% (w/v) dried skimmed milk powder for 1 h, and then incubated overnight at 4 C with relevant primary antibodies diluted in 5% (w/v) milk TBST

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Although ferroptosis has emerged as a potentially valuable target in ovarian-related therapeutic research, current investigations into the regulatory effects of natural products on ferroptosis remain preliminary

[155] In line with this invention either the first, the second or the first and the second domain may comprise a single domain antibody, respectively the variable domain or at least the CDRs of a single domain antibody