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24 Copper homeostasis is tightly regulated in cells To maintain Cu homeostasis at the cellular level, intracellular Cu content is regulated by a complex network of Cu-dependent proteins, including cuproenzymes, Cu chaperones, and membrane transporters

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The resultant files were processed with El-Maven (Elucidata) alongside the Kyoto Encyclopedia of Genes and Genomes (KEGG) 20,21,22 database for metabolite assignment and manual peak integration as previously described using intact mass, isotopic patterns, and an in-house standard library 23

For maximum shelf life and integrity, lyophilized peptides should be stored in a sealed vial at refrigerated or frozen temperatures prior to reconstitution.Preferred storage: 20C (freezer)For long-term preservation and maximum degradation prevention: 80C (ultra-low freezer)Avoid frequent freeze-thaw cycles, exposure to humidity, or light