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doi: 10.3390/agronomy12040813 67 LiJ.ZhaoC.ZhangM.YuanF.ChenM

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When selecting the IMAC resin, we first need to consider the following properties of the resin: Binding capacity of the resin, which impacts the yield of target proteins Bind specificity of the resin, which influences the purity of target proteins Tendency of metal leaching from the matrix Chemical stability of the resin, which impacts the feasibility of resin being adapted in the optimal buffer conditions for the protein Methodology of separation in the application : manual (by batch/gravity flow) or automation (by pressurized flow) Lets look into each of these characteristics

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The FDA isn't bound by the committee's recommendations but typically follows them