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However, higher concentrations (1mL reconstitution) may show slightly slower degradation due to reduced surface area exposure, though the difference is negligible compared to storage temperature compliance

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Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin