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The resulting mixture was incubated at 37 C with continuous shaking for 2 h, followed by centrifugation at 10,000 rpm for 1 h using centrifuge (Centurion Scientific Ltd)

Examples of the microenvironmental conditions influencing the dual effects of Nrf2 in cancer include overexpression of the GSTP1 enzyme, Keap1 mutations, loss of heterozygosity, and hypoxia/reoxygenation

N Engl J Med (2018) 378(10):9319

Melanin is the natural pigment responsible for skin, hair, and eye colour

Additionally, the generation of OH was directly measured by EPR with DMPO as a radical trapping agent

Song, Z., Wang, L., Cao, Y., Liu, Z., Zhang, M., Zhang, Z., Jiang, S., Fan, R., Hao, T., Yang, R., Wang, B., Guan, Z., Zhu, L., Liu, Z., Zhang, S., Zhao, L., Xu, Z., Xu, H., & Dai, G