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Afterward, the membranes were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody and immunoreactive bands were detected by a Fluorchem Imaging System upon staining with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA)

Alpha-lipoic acid supplementation significantly reduces the risk of obesity in an updated systematic review and dose response meta-analysis of randomised placebo-controlled clinical trials

This limitation highlights the critical importance of early intervention during the reversible disease phase

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Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35 + B cell generation