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The primary consideration is measurement precision smaller volumes (1mL) create higher concentrations requiring finer syringe gradations, while larger volumes improve ease of measurement

After centrifugation, the cells were incubated with H 2 DCFDA for 30 min in the dark and washed twice

Skeletal muscle protein balance and metabolism in the elderly

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Moreover, insulin-like growth factor binding protein related proteins (IGFB- PrPs) have been recently characterized, which aid the metabolic effects of the hormone but their role remains unclear [8]