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Although drought and salinity are often grouped as osmotic stresses because both reduce plant water potential and activate conserved responses, such as ABA-mediated stomatal regulation, osmotic adjustment, and antioxidant defenses, their effects arise from distinct soilplant mechanisms that differentially influence nutrient acquisition

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RNA extraction, cDNA library construction, and sequencing Total RNA was isolated from the buckwheat seedling after 3 days of chilling stress using the Trizol reagent kit (Invitrogen, Carlsbad, CA, United States)

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