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After removing the magnetic beads, samples were spun down at 20,000 g for 10 min to remove the remaining beads, and the supernatant was transferred to a scintillation vial with 5 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339)

[DOI] [PubMed] [Google Scholar] Learn DB, Fried VA, Thomas EL

Protein binding may be decreased in the elderly resulting in a larger proportion of the total serum theophylline concentration in the pharmacologically active unbound form

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Collagen helps to neutralize aging factors and improve skin elasticity.

B., Rubin, J