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Each oligo sequence contains an adapter, a barcode to uniquely identify the cell source of RNA, a random tag for RNA identification and quantification, and an oligo-dT primer for mRNA binding

MitoTracker green, MitoSox, and TMRM analysis via flow cytometry Prior to staining with TMRM or MitoSox respective positive controls were prepared either by treating with 1 M Antimycin A (A8674, Sigma Aldrich) for 30 min or 0.5 M FCCP (1528-10, Sanbio) for 20 min