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Briefly, each primary antibody was incubated for 12 h in a humidified chamber at 37 C, followed by detection using the HRP-conjugated secondary antibody and TSA-dendron-fluorophores (Histova, NEON 7-color IHC Kit for cryosection, 1:100), after which the primary and secondary antibodies were thoroughly eluted in stripping solution (Histova, Abcracker) for 60 min at 37 C

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PMID 11935176

This will exaggeratedly raise the content of vitamin C of the juice and will confer it a very nice strong-orange color (depending on the added amount)

In a rat model of CCI, liproxstatin-1 restored dysregulated levels of GPX4 and ACSL4, reduced iron content, diminished spinal cord lipid peroxidation, and prevented mitochondrial changes [23]

10.1038/s41417-020-0182-y 289 LiuY.ZhengY.KaratasH.WangX.FoerchC.LoE