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2025 Jun 18:111614

Figure 2. A 25 kDa protein was expressed in E.coli and purified on an automated system using aone-step automated protocol with ProteIndex HiBond Ni-NTA Agarose 6 Fast Flow resin under native conditions. 50 mL E.coli lysate in 50 mM NaH2PO4, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL/min. The column was then washed with 100 mL 50 mM NaH2PO4, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL/min. The target protein was eluted with 50 mM NaH2PO4, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgents ProteIndex Ni-NTA 6FF (M) and Ni-Sepharose (G) resins

Environmental emissions of elemental and inorganic mercury originate from coal-fired power stations and from plants that discharge mercury-contaminated water

PMC 9392980

Conclusion In this prospective cohort of septic patients, reduced plasma glutamic acid levels at hospital admission were significantly associated with the presence of myelosuppression and increased 30-day mortality