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After the reoxygenation phase, cells were washed with PBS to remove excess fluorescent compounds

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Mechanistically, ferroptosis is mediated by the generation of toxic oxidized lipids, including 4-hydroxynonenal [6], as a result of lipid peroxidation [7]

It also is suggested to improve colitis by decreasing the deleterious metabolite ammonia while ameliorating gut microbiota dysbiosis [342]