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Fully automated two-step assay for detection of metallothionein through magnetic isolation using functionalized -Fe2O3 particles

After washing with PBS, bound FPR1 and CXCR1 were detected with a polyclonal GST antibody, followed by incubation with a horseradish peroxidase-conjugated sheep anti-goat IgG (H + L) (Invitrogen)

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Nakamura, T., Akita, H., Yamada, Y., Hatakeyama, H

The RNA-seq analysis revealed upregulation of apoptosis-related genes (CASP1, CASP4, CASP8, CASP9, CASP10, BAX, TP53, PTEN, and FAS) (Fig