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When exogenous pure compounds were spiked to fresh blood (Sample Type 1), recovery rates were below 100%: NAD + at 92.5%, NADH at 10%, NADP + at 80% and NADPH at 60%

A heatmap was made by using the metric fragments per kilobase of feature per million mapped reads (FPKM) value of each transcript, and genes associated with the selected pathways were prominent on the heatmap