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and the mixture was vortexed and centrifuged (10,000 g, 10 min, 4C) to afford a protein disc at the interface of CHCl 3 and aqueous layers

(2025) 17:e86080

1 H, 15 N HSQC spectra of 100 M 15 N-labeled BRD4 ET in 25 mM Tris pH 7.0, 150 mM NaCl, 1 mM TCEP, 10% D 2 O were recorded in the presence of increasing concentrations of ASXLs peptides