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After culture for 40 h, cells were collected and lysed in 8 ml of hypotonic buffer (10 mM HEPES pH 8.0, 10 mM KOAc, 1.5 mM Mg(OAc) 2 , 2% (v/v) glycerol, 5 mM NDSB-256 and 0.5 mM TCEP, with 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001) per 10 ml of buffer) by a Dounce homogenizer

Although similar pocket sizes of ShHTL1 and ShHTL3 have been predicted by modeling, our data showed that the ligand-binding pocket of ShHTL3 31 was smaller than that of ShHTL1

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85 15 Nov 1997 Rapid City, SD Wernersville, PA 16 Nov 1997 C2 18 Nov 1997 B2 Kaczmarek, Joy E