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The cells were lysed by ultrasound for 20 min at 4 C, followed by centrifugation at 12,000 rpm for 15 min to collect the supernatant

The NO/cGMP/PKG pathway in platelets: the therapeutic potential of PDE5 inhibitors in platelet disorders

Oxidation of methionine residues (+15.99 Da) was included as a common variable modification (up to two per peptide)

Marek GJ, Behl B et al (2010)

Additionally, glutathione directly interacts with toxins to neutralize their harmful effects and facilitates the transportation of these toxins out of cells

The active group of the molecule is represented by the thiol group (-SH) of the cysteine residue (Figure 1) which provides its reductive power