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Unfortunately, MTX can trigger apoptosis in healthy tissues, including the liver (Xiong et al., 2020)

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Plasma was in Eppendorf tubes and flash-frozen in liquid nitrogen prior to storage at -80C

(2010) Quantitative analysis of cellular inflammation after traumatic spinal cord injury: evidence for a multiphasic inflammatory response in the acute to chronic environment Brain 133, 433447 10

The mobile phase consisted of 0.1% formic acid (A) and 0.1% formic acid in acetonitrile (B)