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For DDA measurement, the same LC-gradient was measured with MS1 resolution 60,000, MS1 AGC target 3e6, MS1 max IT 45 ms, scan range 3501400 m / z , MS2 resolution 30,000, MS2 AGC target 1e5, MS2 max IT 54 ms, MS2 top6, MS2 isolation window 1.3 m / z , MS2 scan range 200-2000 m / z , MS2 NCE 28%, and MS2 dynamic exclusion 30 s

2. Gently dab to aid absorption

doi: 10.3390/ijms251810217 112

Briefly, cell extracts were prepared using the RIPA lysis buffer (150 mM sodium chloride, 1% NP-40, 0.1% SDS, 50 mM Tris, pH 7.4) containing 1 mM -glycerophosphate, 2.5 mM sodium pyrophosphate, 1 mM sodium fluoride, 1 mM sodium orthovanadate, and a protease inhibitor (Roche, Cat

No single-receptor or even dual-receptor agonist can replicate this combination, which is why retatrutide produced weight loss numbers that surpassed both Ozempic and tirzepatide in clinical comparisons