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Furthermore, it indirectly regulates the Th17/Treg balance of CD4+ T cells via macrophage interaction, thereby simultaneously remodeling both innate and adaptive immune networks

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Membrane was blocked in 5% (w/v) non-fat dry milk in TBST for 1 h at room temperature, and probed against the anti-glutathione antibody (Virogen, Grater Boston, MA, USA) at the dilution of 11000 in 1% (w/v) non-fat dry milk TTBS over night at 4C, and secondary antibody, goat anti-mouse IgG (Calbiochem, San Diego, CA, USA) conjugated to horseradish peroxidase, at 17500 in 1% (w/v) non-fat dry milk for 1 h at room temperature