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Mayerl C, Lukasser M, Sedivy R, Niederegger H, Seiler R, Wick G

The K NADPH values determined from these fits were 7 0.1 M (C159S data) and 20 1 M (U597S data)

PoeweWStankovicIHallidayGMeissnerWGWenningGKPellecchiaMTet al

Recombinant N-terminally MRGS(H) 6 -tagged wild-type and mutant ScGrx7 and HsGrx5 C122S (without their N-terminal targeting sequences 19 ) were purified after lysozyme treatment and sonication by Ni-NTA affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl, and 50 mM sodium phosphate, pH 8.0 11,19,25,42

GHK-Cu works through copper-dependent enzyme activity, extracellular matrix signaling, integrin pathways, MAPK/ERK cascades, and direct gene expression modulation