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Static modifications for N -termini and lysine residues corresponding to the 6-plex TMT tag (+229.1629 Da) were set

We then added the PKA pathway inhibitor H89 to mimic the effect of A2ARi in compromising activated T cell survival (Supplemental Figure 6, G and H) while upregulating GSH metabolic genes ( gclc , gclm , and gpx4 ) (Supplemental Figure 6I)

Simultaneously, metformin activates the LKB1/AMPK signaling pathway, leading to the inhibition of aerobic glycolysis in cells, as well as the insulin-like growth factor-1(IGF-1) signaling pathway and mTOR pathway in EC cells

The lysate was then centrifuged at 20,000 g at 4 C for 15 min, the supernatant was collected to remove any cell debris

Therefore, the screening of functional food factors with antihypertensive effects is critical for the prevention and treatment of hypertension