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The strips were then loaded onto 12% (w/v) SDS-PAGE gels and sealed with 0.5% agarose

Thus, whereas salvage pathways might provide a sufficient source of DNA precursors for repair and perhaps for mitochondrial DNA replication, they generally will not support S phase genome replication (Iwasaki et al., 1997

& Wakeham, S

J Biol Chem 277:2694426949 Couturier J, Przybyla-Toscano J, Roret T, Didierjean C, Rouhier N (2015) The roles of glutaredoxins ligating Fe-S clusters: sensing, transfer or repair functions

Single-point energies were calculated on the optimized geometries using the B97X-D hybrid functional 87 and the 6-311 + G(2d,p) basis set with ultrafine integration grids for the QM part and the ff14SB, gaff2 and TIP3P force fields for the MM part (protein, substrate and water, respectively) with electrostatic embedding