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This cuproenzyme activation directly drives antioxidant defence, collagen crosslinking, and mitochondrial respiration efficiency distinguishing GHK-Cu from purely receptor-mediated peptide mechanisms

The latter observation also indicates that cell death caused by prolonged ER stress is not linked to H 2 O 2 production (Haynes et al , 2004), as HGT1 ire1 cells did not generate H 2 O 2 when exposed to GSH, but were much more sensitive to its toxicity

CCK8 was added and incubated for another 2 h, and then the absorbance at a wavelength of 450 nm was measured by Multiskan FC (Thermo Scientific, USA) simultaneously every day

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Cdk5: cyclin-dependent kinase 5