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Results Identification of HK2 as a suppressor of TNF-induced cell death mediated by RIPK1 In our previous screening 4 , which is aimed to investigate the role of metabolic pathways in TNF-induced cell death, we found that knockdown of Hk2 sensitized primary hepatocytes to cell death induced by TNF and cycloheximide (CHX), a combination known to induce caspase 8-mediated apoptosis and independent of RIPK1 kinase activity 41

The protein sample was then diluted by adding 100 mM TEAB to urea concentration less than 2 M

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doi: 10.1530/JOE-13-0285