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Description

Nature 560 , 649654 (2018)

Yao, L., Xue, X., Yu, P., Ni, Y

After 10 hours (microinjected embryo experiments) or 16 hours (non-microinjected embryo experiments) in BO-IVF medium (IVF Biosciences, Falmouth, UK), IVF embryos were denuded from cumulus cells by vortexing for 5 min in BO-Wash medium (IVF Bioscience, Falmouth, UK) and cultured up to 7.5 d in BO-IVC medium (IVF Biosciences, Falmouth, UK) at 38.5 C, 6% CO 2 , and 6% O 2