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Phase separation was achieved by centrifugation at 12,000 rpm for 15 min at 4 C

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The NOAEL was 300 ppm (equivalent to 7.5 mg/kg bw per day) on the basis of increased relative liver weights, increased alanine aminotransferase and alkaline phosphatase activities, and hepatocellular degeneration in females at 1000 ppm (equivalent to 25 mg/kg bw per day)

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