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Regular monitoring, lab testing, and periodic reassessment are essential to reduce potential harm and identify early warning signs

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After 24 h, total protein extracts were obtained from whole flies homogenized in standard lysis buffer (20 mM Tris-Cl (pH 7.5), 200 mM NaCl, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40 and EDTA-free Complete Protease Inhibitor Cocktail (Roche Diagnostics))

sativa extract reduced oxidant parameters, such as malondialdehyde (MDA), glutathione (GSH) and skin catalase (CAT) and increased antioxidant-supporting enzyme parameters, such as glutathione peroxidase (GPx) and SOD levels, markedly compared with controls

Its finally getting better but still a large red area and itchiness