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This article demonstrates enhanced immunization and protection against influenza-ova infection via pulmonary administration of lymph node-targeted antigens in nanoparticles with CpG relative to administration of soluble antigens with CpG

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Berndt C, Christ L, Rouhier N, Mhlenhoff U

Solvent A was prepared by mixing 99 mL of Milli-Q (Millipore) water and 1 mL of 1 mol/L ammonium bicarbonate and was adjusted to a pH of 9.2 with 25% ammonium hydroxide (~0.1 mL)