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Correlations among TGF- , HGF and stem cell markers in single-cell qRTPCR analysis using freshly resected glioblastoma tissues Finally, we expanded our study to analyze expression profiles of TGF- , HGF , and a panel of genes essential for pluripotency in the CD45 - /CD31 - presumptive tumor cell population isolated from freshly resected tumor tissue of 6 glioblastoma patients at diagnosis