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The mass spectrometry used was Q Exactive HF (Thermo Fisher Scientific, San Jose, CA), and scanned from 70 to 1000 m/z with switching polarity

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[21], the presence of an elevated level of antioxidant enzyme in the host is to provide a cellular protective role to oppose the oxidative stress generated by parasitic invasion

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MT-II was designed and characterised through the systematic melanocortin peptide structure-activity relationship programme in Victor Hrubys laboratory at the University of Arizona , the same programme that produced MT-1 , with the cyclisation strategy introduced to further constrain the pharmacophore into a receptor-binding conformation that simultaneously increases potency, metabolic stability, and the receptor subtype selectivity profile that distinguishes MT-II from the linear tridecapeptide MT-1

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