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doi: 10.1007/s11010-010-0510-x 134 GangulyKRauthSMarimuthuSKumarSBatraSK

So lets start by clearing the air

centrifugation is performed at 4C, 10,000 g for 10-15 min, and the supernatant is extracted for the subsequent reduced glutathione assay

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In IR, enhancing the -CT structural flexibility by the insertion of four glycines in the N-terminal part of -CT increases insulin-binding affinity 15 , suggesting that the origin of negative cooperativity between IR and IGF1R may be similar